Bio-Rad Western Blot TBST: Unlocking the Power of Fluorescent Multiplexing

When it comes to western blotting, preserving your precious sample is crucial for accurate results. Bio-Rad's StarBright Blue 700 Secondary Antibodies and ChemiDoc MP Imaging System are designed to aid fluorescent multiplexing, enabling direct protein abundance comparisons and normalization against a control. This approach is more accurate, easier, and time-saving than traditional methods.

Fluorescent western blotting allows for the detection of multiple proteins in a single sample using highly specific and sensitive fluorescent antibodies. Bio-Rad's Western Blot Doctor, a self-help guide, can help troubleshoot common issues and ensure successful western blots. For beginners, our introduction to western blotting guide provides step-by-step instructions on this essential technique.

In simple terms, western blotting, also known as immunoblotting or protein blotting, is used to detect the presence of a specific protein in a complex mixture extracted from cells or tissue. It's a fundamental technique in cell and molecular biology.

To achieve confident detection of phosphorylated proteins, it's essential to dilute horseradish peroxidase (HRP) conjugated secondary antibodies in full-strength EveryBlot Blocking solution. Bio-Rad recommends the use of Tris-buffered saline (TBS) over phosphate-buffered saline (PBS) based buffers for this purpose.

For western blot detection of phosphorylation events, it's crucial to rinse the blot five times with TBST for 5 minutes each. This ensures optimal binding of antibodies and minimizes non-specific binding.

In conclusion, Bio-Rad's western blot solutions, including StarBright Blue 700 Secondary Antibodies and ChemiDoc MP Imaging System, are designed to simplify and improve western blotting workflows. By following these guidelines and using the right tools, researchers can achieve confident detection of phosphorylated proteins and unlock the power of fluorescent multiplexing.

Bio rad western blot tbst